Tag: DNA barcode

Three days ago, bored with keep writing down uncountable unidentified spp., I decided to do this. 44 fish specimens including two eggs and 42 larvae were dissected for DNA extracting (Sangon DNA extract kit). All but one specimen were successfully amplified in the following PCR (TaKaRa LA Taq kit)....

搁置了一段时间后，今天（周六）拖了Kaiyue Zhang去实验室将上次失败的试验继续搞起：使用新的引物对上次提取的DNA进行扩增。 直接说结果：比上次更失败，一点儿渣都没有扩出来。...

最近的两周里，在好基友Kaiyue Zhang的鼎力帮助下，我们开始了对福尔马林固定的仔稚鱼样品的分子生物学试验，以期用分子生物学方法鉴定出疑难种，并为以后的试验打下基础。从零开始克服各种试验设备条件的困难就不说了，做的过程还算可以，结果有点不堪。...